Mammalian fucosidases. 2. α-l-Fucosidase
نویسندگان
چکیده
منابع مشابه
In vitro and in vivo comparative and competitive activity-based protein profiling of GH29 α-l-fucosidases.
GH29 α-l-fucosidases catalyze the hydrolysis of α-l-fucosidic linkages. Deficiency in human lysosomal α-l-fucosidase (FUCA1) leads to the recessively inherited disorder, fucosidosis. Herein we describe the development of fucopyranose-configured cyclophellitol aziridines as activity-based probes (ABPs) for selective in vitro and in vivo labeling of GH29 α-l-fucosidases from bacteria, mice and ma...
متن کاملMammalian fucosidases. 1. The synthesis of substrates and inhibitors.
of radioautographic techniques (Logan, Ficq & Errera, 1959). This permits the study of incorporation experiments in individual nuclei but does not give any information about the quantitative chemical changes in the nuclei. It is of interest to know whether there are any common biochemical changes in the nuclei as a result of feeding the two carcinogens. However, the two types of nuclear prepara...
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This paper describes the discovery of novel α-L-fucosidases and evaluation of their potential to catalyse the transglycosylation reaction leading to production of fucosylated human milk oligosaccharides. Seven novel α-L-fucosidase-encoding genes were identified by functional screening of a soil-derived metagenome library and expressed in E. coli as recombinant 6xHis-tagged proteins. All seven f...
متن کاملSynthesis of fucosyl-N-acetylglucosamine disaccharides by transfucosylation using α-L-fucosidases from Lactobacillus casei.
AlfB and AlfC α-l-fucosidases from Lactobacillus casei were used in transglycosylation reactions, and they showed high efficiency in synthesizing fucosyldisaccharides. AlfB and AlfC activities exclusively produced fucosyl-α-1,3-N-acetylglucosamine and fucosyl-α-1,6-N-acetylglucosamine, respectively. The reaction kinetics showed that AlfB can convert 23% p-nitrophenyl-α-l-fucopyranoside into fuc...
متن کاملThe gene of an archaeal α-l-fucosidase is expressed by translational frameshifting
The standard rules of genetic translational decoding are altered in specific genes by different events that are globally termed recoding. In Archaea recoding has been unequivocally determined so far only for termination codon readthrough events. We study here the mechanism of expression of a gene encoding for a alpha-l-fucosidase from the archaeon Sulfolobus solfataricus (fucA1), which is split...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1961
ISSN: 0006-2936
DOI: 10.1042/bj0800435